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目的 :测定大黄降脂粉中大黄酸、大黄素、丹参酮ⅡA 的含量。方法 :高效液相色谱法 ,KromasalODS柱。甲醇 - 0 1%磷酸 (2 6 5∶35 )为流动相 ,检测波长 :2 5 4nm ,柱温 :45℃。结果 :可同时测定大黄酸、大黄素、丹参酮ⅡA 的含量。其浓度分别在 1 2 2～ 5 5 5 ,2 2 5～ 10 73 ,0 33～ 1 2 3μg·mL-1范围内与峰面积呈线性关系 ,平均回收率 (n =3)分别为 10 4 2 % (RSD =2 5 % ) ,96 5 % (RSD =2 0 % ) ,95 2 % (RSD =2 8% )。结论 :方法简便、快速准确 ,可作为样品的检测方法。
Objective : To determine the content of rhein, emodin and tanshinone IIA in Rhubarb lipid-lowering powder. Method: HPLC, Kromasal ODS column. Methanol - 0 1% phosphoric acid (2 6 5:35) as mobile phase, detection wavelength: 254 nm, column temperature: 45°C. Results : The content of rhein, emodin and tanshinone IIA can be measured simultaneously. The linear relationship between the concentration and the peak area was in the range of 152-525, 252-107, and 333-132 μg·mL-1. The average recovery rate (n = 3) was 10 4 respectively. 2% (RSD = 25%), 965% (RSD = 20%), 95 2% (RSD = 28%). Conclusion : The method is simple, rapid and accurate. It can be used as a sample detection method.