探索体外培养兔眼 Tenon囊成纤维细胞的简便方法 ,把兔结膜和结膜下组织剪碎后直接接种在培养瓶中 ,加入 DMEM培养液进行培养 ,省略胰蛋白酶消化过程或者组织块干固贴壁过程。此方法简单而且有效 ,本文对此进行了详细的介绍 ,同时还介绍了如何分离培养时混杂生长的结膜上皮细胞 ,并且对体外培养的兔 Tenon囊成纤维细胞的生长速度进行了研究 To explore a simple method of culturing Tenon’s capsule fibroblasts in vitro, the rabbit’s conjunctiva and subconjunctival tissue were cut and directly inoculated in culture flasks, then cultured in DMEM medium, omitting the process of trypsin digestion, process. This method is simple and effective, this article describes in detail, but also describes how to separate and culture mixed conjunctival epithelial cells, and cultured in vitro rabbit Tenon cyst fibroblasts growth rate were studied