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目的观察金银花和山楂果核挥发油体外抗菌效果,为实际应用提供科学依据。方法采用稀释法对两种植物挥发油体外最小抑菌浓度和最小杀菌浓度进行了测定。结果金银花挥发油对革兰阴性菌株的MIC50和MIC90值均≥128μg/ml,对金黄色葡萄球菌MIC90值≥128μg/ml、MIC50值为64μg/ml。山楂果核挥发油对所有试验菌株的MIC50值在16～64μg/ml范围;MIC90值在64～128μg/ml。山楂果核挥发油作用15 min,对大肠杆菌和金黄色葡萄球菌MBC值在32～64μg/ml范围;金银花挥发油作用60 min,对上述两种细菌MBC值为64μg/ml。结论两种植物挥发油对试验菌株都具有良好的体外抗菌效果,但其MIC和MBC值均明显高于两种对照药物。
Objective To observe the antibacterial effect of volatile oil from honeysuckle and hawthorns nucleus in vitro and provide scientific basis for its practical application. Methods The minimum inhibitory concentrations and minimum bactericidal concentrations of volatile oil in two plants were determined by dilution method. Results The MIC50 and MIC90 values of volatile oil of honeysuckle were all higher than 128μg / ml for Gram-negative strains, and the value of urea was ≥ 128μg / ml against Staphylococcus aureus, the MIC50 was 64μg / ml. The MIC50 of hawthorn kernel volatile oil in all tested strains was in the range of 16-64μg / ml; the MIC90 was in the range of 64-128μg / ml. The volatile oil of hawthorn nucleus was applied for 15 min, the MBC value of Escherichia coli and Staphylococcus aureus was in the range of 32-64 μg / ml, the volatile oil of honeysuckle was 60 min, and the MBC value of the two bacteria was 64 μg / ml. Conclusion The volatile oils of two plants have good antibacterial activity in vitro, but their MIC and MBC values are significantly higher than the two control drugs.