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目的:研究胰腺癌细胞基因组范围内的纯合性缺失和杂合性缺失(loss of heterozygosity,LOH).方法:应用高密度单核苷酸多态性芯片和专用分析软件,检测17种胰腺癌细胞株基因组范围内的纯合性缺失和LOH,并筛选可能与胰腺癌发生、发展有关的基因区域,用PCR验证纯合性缺失.结果:经过PCR验证,26个区域确实为纯合性缺失,芯片的准确度为83.9%(26/31).这些缺失区域中,平均每个区域只涉及1.29个基因.每一种细胞都有不同程度的LOH;不同染色体臂出现LOH频率不同,出现频率最高的为染色体臂9p和18q,均为94.1%.结论:胰腺癌全基因组范围内出现多处LOH和纯合性缺失,这些区域可能含有新抑癌基因.
OBJECTIVE: To study the homozygous deletion and loss of heterozygosity (LOH) in the genome of pancreatic cancer cells.Methods: High-density single nucleotide polymorphisms (SNPs) and special analysis software were used to detect 17 kinds of pancreatic cancer Homozygous deletion and LOH in the genome of the cell strain, and screening possible gene regions related to the occurrence and development of pancreatic cancer, and using PCR to verify the homozygous deletion.Results: After PCR validation, 26 regions were indeed homozygous deletion , And the accuracy of the chip was 83.9% (26/31) .In these deletion regions, only 1.29 genes were involved in each region on average. Each cell had different degrees of LOH. The frequencies of LOH in different chromosome arms were different The highest chromosome 9p and 18q were 94.1% .Conclusion: There are many LOH and homozygous deletions in the whole genome of pancreatic cancer, and these regions may contain new tumor suppressor genes.