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采用鼻腔喷雾法(CCID50=105.7)研究了SARS冠状病毒(SARS-CoV)对成年和幼年布氏田鼠的感染效果.成年动物攻毒后出现死亡,表现为口鼻有出血,肠道出血;肺组织呈出血性间质性肺炎改变,肝、脾、肾、胰腺组织均呈淤血性改变;存活动物肺组织呈间质性肺炎,局灶出血及肺气肿改变,其他脏器未见明显病变.幼年动物攻毒后未见死亡但行动较为迟缓,主要脏器未见明显异常;早期肺组织有局限性肺炎改变,且病毒分离为阳性;同居对照组的一只动物有肺组织局灶性肺炎.结果表明,SARS-CoV可以很强地感染布氏田鼠;成年布氏田鼠比幼年动物对SARS-CoV更敏感;布氏田鼠有望成为一种比较理想的小型SARS动物模型。
The infection effect of SARS coronavirus (SARS-CoV) on adult and young Brandt’s vole (Microtus brandti) was studied by nasal spray method (CCID50 = 105.7) .After death in adult animals, the animals died of bleeding from the nose and mouth and intestinal bleeding. Tissue was hemorrhagic interstitial pneumonia changes, liver, spleen, kidney and pancreas showed congestive changes; survivors showed interstitial pneumonia in the lung tissue, focal hemorrhage and emphysema changes, no significant lesions in other organs No deaths were observed in juvenile animals, but their behaviors were relatively slow. There were no obvious abnormalities in the major organs. Localized pneumonia changed in the early lung tissues and the virus was isolated as a positive one. Cohabitation control animals had focal lung tissue Pneumonia.The results showed that SARS-CoV could be strongly infected with Brandt’s vole (Microtus brandti). Adult Brandt’s vole (Microtus brandti) was more susceptible to SARS-CoV than young animal and the Brandt’s vole (Microtus brandti) was expected to become an ideal small animal model of SARS.