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目的 :对比观察滋补肾阴方与温补肾阳方对卵巢切除所致骨质疏松大鼠白细胞介素 1(IL 1)和白细胞介素 6 (IL 6 )活性的影响。方法 :将 5 5只雌性大鼠随机分为正常对照组、假手术组、卵巢切除组、滋阴组和补阳组。于卵巢切除 1周后 ,给大鼠灌胃给药 ,连续给药 3个月。采用骨组织形态计量学方法对大鼠胫骨进行形态计量 ;分别采用小鼠胸腺细胞检测法和IL 6依赖株细胞增殖反应检测法测定IL 1和IL 6的活性。结果 :卵巢切除 3个月后 ,大鼠胫骨骨小梁体积百分比 (TBV % )明显降低 ,骨小梁吸收表面百分比(TRS % )和骨小梁形成表面百分比 (TFS % )皆显著增高。同时 ,IL 1和IL 6活性亦显著增高。给大鼠灌服滋补肾阴方和温补肾阳方后 ,均能使上述指标发生逆转 ,但程度有所不同 ,温补肾阳方的效果要明显优于滋补肾阴方。结论 :温补肾阳方对IL 1、IL 6活性的抑制作用明显强于滋补肾阴方 ,这是其对卵巢切除所致大鼠骨质疏松的预防作用之所以优于滋补肾阴方的机理之一。
Objective : To observe the effects of Nourishing Kidney Yin Decoction and Wen Bushen Yang Yang on the activity of interleukin 1 (IL 1) and interleukin 6 (IL 6) in rats with osteoporosis induced by ovariectomy. Methods: Fifty-five female rats were randomly divided into normal control group, sham operation group, oophorectomy group, Ziyin group and Buyang group. One week after ovariectomy, rats were intragastrically administered for 3 months. Bone histomorphometry was used for morphometric measurement of rat tibia; IL 1 and IL 6 activity were measured by mouse thymocyte assay and IL 6 dependent cell proliferation assay. RESULTS: After 3 months of ovariectomy, the percentage of trabecular bone volume (TBV%) decreased significantly, and the percent of trabecular bone surface (TRS%) and the percentage of trabecular bone formation surface (TFS%) were both significantly higher. At the same time, the activity of IL 1 and IL 6 also increased significantly. After the rats were given tonic nourishing kidney yin and warming up the kidney yang, all these indexes could be reversed, but the degree was different. The effect of warming and supplementing kidney yang was obviously better than nourishing kidney and yin. Conclusion: The suppressive effect of Wenbu Shenyang Recipe on the activity of IL-1 and IL-6 is obviously stronger than that of nourishing Kidney Yin, which is the mechanism of its preventive effect on ovariectomy-induced osteoporosis in rats. one.