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目的了解来自亚洲的副溶血弧菌临床菌株的群组结构和克隆复合体构成。方法在pub MLST公共数据中筛选具有完整ST型及p ST型的来自亚洲的副溶血弧菌临床菌株数据,进行亚群分析和克隆复合体分析,并分别构建基于ST型和p ST型的最小生成树。结果从数据库中共筛选到具有ST型及p ST型的来自亚洲的副溶血弧菌临床菌株数据341条,共含有157个ST型,以ST3型为最多。其中有214条菌株数据来自中国(包括港台地区),覆盖了133个ST型,其中仍以ST3型为最多。利用e BURST软件对数据进行分析,共发现了17个组,94个单体。STRUCTURE软件分析显示,来自亚洲的副溶血弧菌临床菌株的适宜亚群数为7,各亚群内的样品平均距离为0.9113。结论来自亚洲的副溶血弧菌临床菌株具有高度多态性,可细分为7个亚群,MLST分型时以ST3型为多,AA-MLST分型时以p ST2型为主。
Objective To understand the group structure and clonal complex formation of clinical strains of Vibrio parahaemolyticus from Asia. Methods The clinical strains of Vibrio parahaemolyticus with complete ST and p ST types were screened from pub MLST public data for subpopulation analysis and clonal complex analysis and to construct the minimal Spanning Tree. Results A total of 341 strains of Vibrio parahaemolyticus strains with ST and p ST types were screened from the database. There were 157 ST types, most of which were ST3 type. Among them, 214 strains were from China (including Hong Kong and Taiwan), covering 133 STs, of which ST3 was the most common. Using eBURST software to analyze the data, a total of 17 groups and 94 monomers were found. STRUCTURE software analysis showed that the appropriate subpopulation of clinical strains of V. parahaemolyticus from Asia was 7 and the average distance of samples within each subpopulation was 0.9113. Conclusion The clinical isolates of Vibrio parahaemolyticus from Asia are highly polymorphic and can be subdivided into seven subgroups. Most ST3-type strains are found in MLST typing and p ST2-type in AA-MLST classification.