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结合ＰＣＲ技术和变性剂的作用，扩增出内皮型一氧化氮合成酶（ｅＮＯＳ）８０１～９０２ＡＡ区Ｇ＋Ｃ超含量（７１．５％）编码基因；同时研究了各种变性剂及其不同浓度对扩增的影响。结果显示，ＤＭＳＯ、吐温２０可促进该序列特异性扩增，其中ＤＭＳＯ在１．５％～２％、吐温２０在０．１５％浓度时效果最佳。扩增产物为建立ｅＮＯＳ检测试剂盒作了必要准备。本实验证明，变性剂可明显改善Ｇ＋Ｃ超含量ＤＮＡ序列的特异性扩增，这对临床应用ＰＣＲ技术扩增Ｇ＋Ｃ高或超含量基因片段具有重大的参考意义
The gene encoding G + C superfamily (71.5%) was amplified from 801 to 902AA of endothelial nitric oxide synthase (eNOS) by polymerase chain reaction (PCR) and denaturing agent. At the same time, various denaturants and different concentrations of Effect of amplification. The results showed that DMSO and Tween 20 could promote the sequence-specific amplification, DMSO was at 1.5% ~ 2% and Tween 20 at 0.15% had the best effect. Amplification products for the establishment of eNOS detection kit made the necessary preparations. This experiment proved that denaturant can significantly improve the G + C content DNA sequence specific amplification, which is of clinical application of PCR amplification of G + C gene content of high or super-significant reference