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观察B16F10/IL-21-gpi瘤苗对小鼠黑色素瘤治疗作用,并探讨其作用机制。以灭活的B16F10/IL-21-gpi瘤苗治疗黑色素瘤小鼠,经观察小鼠肿瘤生长大小、生存期长短判断其治疗肿瘤效应。以双抗夹心ELISA法检测小鼠脾细胞分泌的IL-4、IFN-γ和RT-PCR法检测肿瘤组织中IL-21的表达状况;同时以流式细胞仪检测小鼠脾细胞、肿瘤组织中和肿瘤引流淋巴结Treg的比例。结果显示,B16F10/IL-21-gpi瘤苗治疗鼠未成瘤率增高,肿瘤生长变慢;GPI锚定的IL-21瘤苗可很好地在肿瘤局部表达,Treg比例减少,脾细胞分泌的IFN-γ有明显地升高。该结果表明B16F10/IL-21-gpi瘤苗具有良好的治疗鼠黑色素瘤作用,并可能通过表达IL-21诱导IFN-γ分泌增加而抑制Treg细胞的减少达到抗肿瘤效应。
To observe the therapeutic effect of B16F10 / IL-21-gpi tumor vaccine on mouse melanoma and to explore its mechanism. The inactivated B16F10 / IL-21-gpi tumor vaccine was used to treat melanoma mice. The tumor growth was observed and the survival of mice was observed. IL-4, IFN-γ and RT-PCR were used to detect the expression of IL-21 in the tumor tissue by double-antibody sandwich ELISA. Meanwhile, the spleen cells, tumor tissues Neutralize the proportion of tumor-draining lymph node Tregs. The results showed that the rate of tumorigenesis was not increased in B16F10 / IL-21-gpi tumor-treated mice, and the tumor growth was slow. The GPI-anchored IL-21 vaccine was well localized in the tumor, with a decrease in the proportion of Tregs, IFN-γ was significantly increased. The results indicate that the B16F10 / IL-21-gpi tumor vaccine has a good therapeutic effect on murine melanoma and may achieve anti-tumor effect by inhibiting the decrease of Treg cells by inducing the increase of IFN-γ secretion by expressing IL-21.