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目的:探讨益气活血方(宁心痛颗粒)调节巨噬细胞(MΦ)清道夫受体A(SRA)、CD36干预人类单核细胞株(THP-1)源性MΦ泡沫化的机制。方法:采用MTT法筛选出宁心痛颗粒含药血清作用于THP-1源性MΦ的最适合剂量;建立THP-1源性MΦ泡沫化模型,用该最适合剂量的宁心痛颗粒含药血清进行干预,Western blot法检测干预后细胞脂蛋白受体SRA、CD36蛋白表达水平,与空白组(THP-1细胞组)、THP-1源性MΦ组、模型组(泡沫细胞组)进行比较。结果:宁心痛颗粒10倍剂量含药血清作用下的THP-1源性MΦ存活率最高;与空白组比较,THP-1源性MΦ组、模型组及宁心痛颗粒10倍剂量含药血清组SRA、CD36蛋白表达均升高(P<0.01);宁心痛颗粒10倍剂量含药血清组SRA、CD36蛋白表达水平明显低于模型组(P<0.01)。结论:益气活血方能够下调THP-1源性MΦ的SRA、CD3蛋白表达,从而阻抑泡沫细胞的形成。这可能是该药干预动脉粥样硬化易损斑块的分子机制之一。
OBJECTIVE: To investigate the mechanism of Yiqi Huoxue Fang (NingXinTong Granule) regulating macrophage (MΦ) scavenger receptor A (SRA) and CD36 intervention in THP-1-derived MΦ foamy. Methods: MTT method was used to select the most suitable dosage of Ningxintong granule-containing serum for THP-1-derived MΦ. THP-1-derived MΦ foam model was established, and the most suitable dose of Ningxintong granule- The levels of SRA and CD36 protein in the lipoprotein receptor were detected by Western blot and compared with the blank group (THP-1 cell group), the THP-1-derived MΦ group and the model group (foam cell group). Results: The THP-1-derived MΦ had the highest survival rate under the action of 10 times-dose-containing serum of NingXinTong granules. Compared with the blank group, THP-1-derived MΦ group, model group and NingXueTong granules (P <0.01). The expression levels of SRA and CD36 in serum of Ningxintong Granules group at 10 times dose were significantly lower than that of model group (P <0.01). Conclusion: Yiqi Huoxue Recipe can down-regulate the expression of SRA and CD3 protein in THP-1-derived MΦ, thereby inhibiting the formation of foam cells. This may be one of the molecular mechanisms by which the drug interferes with vulnerable plaques in atherosclerosis.