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用流式免疫荧光、聚合酶链反应（ＰＣＲ）及单链构象多态（ＳＳＣＰ）技术对３３例膀胱移行细胞癌患者的Ｐ５３基因表达及突变、人乳头状瘤病毒（ＨＰＶ）感染进行了研究。结果Ｐ５３基因表达阳性１７例（５１．５％），Ｇ１＋Ｇ２级的阳性率显著低于Ｇ３级，并倾向分布于浸润型（Ⅰ）膀胱癌；Ｐ５３基因突变９例（皆为Ｐ５３阳性表达），倾向分布于Ｇ３级和浸润型膀胱癌。ＨＰＶ阳性１３例（３９．４％），Ｇ１＋Ｇ２级的阳性率显著高于Ｇ３级，并倾向分布于浅表型膀胱癌。认为Ｐ５３基因变化和ＨＰＶ感染在膀胱癌的发生中可能起协同作用。
The expression of P53 gene and mutation and human papilloma virus (HPV) infection in 33 cases of bladder transitional cell carcinoma were studied by flow cytometry, immunofluorescence, polymerase chain reaction (PCR) and single strand conformation polymorphism (SSCP) . Results The positive rate of P53 gene expression was 51.5%. The positive rate of G1 + G2 was significantly lower than that of G3. The positive rate of P53 gene expression was in invasive (Ⅰ) bladder cancer. P53 gene mutation was found in 9 cases (all P53 positive) Tendency distribution in G3 grade and invasive bladder cancer. HPV positive in 13 cases (39.4%), G1 + G2 grade positive rate was significantly higher than the G3 level, and tend to be distributed in superficial bladder cancer. It is thought that P53 gene mutation and HPV infection may play a synergistic role in the occurrence of bladder cancer.