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采用营养液培养试验,通过添加不同浓度(0.05、0.1、0.2、0.3mmol·L-1)的硝普钠(SNP)作为NO供体,研究外源NO对NO3-胁迫下黄瓜幼苗生长及叶片抗氧化酶活性的影响.结果表明:140mmol·L-1NO3-胁迫下,外加0.1mmol·L-1SNP处理1d和7d后,黄瓜幼苗叶片中SOD、CAT和APX活性显著升高;MDA含量显著降低,可溶性蛋白含量增加,说明外加0.1mmol·L-1SNP增强了黄瓜幼苗对活性氧的清除能力,降低了膜脂过氧化程度,幼苗生长势增加,对高浓度NO3-胁迫的抗性增强;当SNP浓度达0.3mmol·L-1处理7d后,SOD、POD、CAT和APX活性均开始降低,MDA含量增加,黄瓜幼苗受害加重.外加一定浓度(0.1～0.2mmol·L-1)的外源NO可缓解NO3-胁迫对黄瓜幼苗的影响.
Nutrient solution culture experiments were conducted to study the effects of exogenous nitric oxide on the growth of cucumber seedlings and on the growth of leaves under different concentrations of NO (superscript -) SNP (0.05, 0.1, 0.2 and 0.3 mmol·L -1) The results showed that the activity of SOD, CAT and APX in cucumber seedling leaves increased significantly and the content of MDA decreased significantly after treated with 0.1mmol·L-1 SNP for 1 and 7 days under the stress of 140mmol·L-1NO3- , Soluble protein content increased, indicating that 0.1mmol·L-1SNP enhanced cucumber seedling ability to scavenge reactive oxygen species, reduced lipid peroxidation, seedling growth potential increased resistance to high concentrations of NO3-stress; when The activity of SOD, POD, CAT and APX began to decrease after treated with 0.3mmol·L-1 SNP, the content of MDA increased, and the damage of cucumber seedlings was aggravated.The concentration of 0.1 ~ 0.2mmol·L-1 exogenous NO can alleviate the effect of NO3-stress on cucumber seedlings.