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[目的]以健康人和慢性乙型肝炎患者作为对照,通过流式细胞术及细胞因子染色方法阐述不同分期的肝硬化患者外周血单核细胞的表型特点。[方法]采用流式细胞术检测外周血单核细胞表型(CD16,CD80及HLA-DR)。[结果]肝硬化患者外周血单核细胞及其亚型(CD14+CD16+单核细胞)频率均增加,HLA-DR表达水平降低。[结论]肝硬化患者病情越重,则单核细胞及CD14+CD16+单核细胞频率越高,活化程度越低。
[Objective] To compare the phenotypic characteristics of peripheral blood mononuclear cells in patients with different stages of liver cirrhosis by using the methods of flow cytometry and cytokine staining in healthy and chronic hepatitis B patients. [Methods] Peripheral blood mononuclear cell phenotypes (CD16, CD80 and HLA-DR) were detected by flow cytometry. [Results] The frequency of peripheral blood mononuclear cells and its subtypes (CD14 + CD16 + monocytes) increased and the expression of HLA-DR decreased in patients with cirrhosis. [Conclusion] The more severe the disease in patients with cirrhosis, the higher the frequency of monocytes and CD14 + CD16 + monocytes, the lower the activation degree.