本室于1986年对传统测定人红细胞胰岛素受体的Gambhir法作了修正,结果表明操作简便,显著地减少了实验误差。本法用肝素抗凝静脉血,分离红细胞,将红细胞悬浮于缓冲液中(8.0×10~(?)RBC/ml),在100μl细胞悬液中加入20μl不同浓度的标准胰岛素和80μl(40pg)A14-~(125)I胰岛素,4℃温育24小时,用PEG作分离剂,测沉淀的放射性,碘标 In 1986, we revised Gambhir’s traditional method of determining human erythrocyte insulin receptor. The results showed that the method was simple and easy to operate, and significantly reduced the experimental error. This method uses heparin anticoagulant venous blood to separate erythrocytes, suspend erythrocytes in buffer (8.0 × 10 ~ (-) RBC / ml), add 20μl different concentrations of standard insulin and 80μl (40pg) to 100μl cell suspension, A14- ~ (125) I insulin, incubated at 4 ℃ for 24 hours, with PEG as a separation agent, measured precipitation radioactive, iodine