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目的本研究通过T细胞输注方法阻断程序凋亡因子-1(programmed death-1,PD-1)抑制性信号在CD8+T细胞上的表达,来治疗鼠获得性免疫缺陷综合征(MAIDS)相关性B细胞淋巴瘤。方法为了选择性阻断PD-1抑制性信号通路在CD8 T细胞中的表达,采用B6.PD-1-/-小鼠作为CD8 T细胞的供体,与来自野生型B6小鼠的CD8 T细胞做比较。定期测量肿瘤直径大小,来检测PD-1-/-和野生型的CD8 T细胞抗肿瘤功能。结果我们的研究结果发现未接受CD8 T细胞输注的Rag-1-/-受体小鼠,肿瘤持续增大。接受B6.PD-1-/-CD8 T细胞输注的受体小鼠肿瘤缩小/消失的时间和速度均比接受野生型B6 CD8 T细胞输注的受体小鼠显著性缩短和加快。结论阻断CD8 T细胞中的PD-1抑制性信号通路能够增强保护性CD8 T细胞抗肿瘤的功能,可达到控制和治疗逆转录病毒感染导致MAIDS相关肿瘤的发生和发展。
OBJECTIVE: In this study, mouse immunodeficiency syndrome (MAIDS) was treated by blocking the expression of programmed death-1 (PD-1) inhibitory signal on CD8 + T cells by T cell infusion Related B Cell Lymphoma. Methods To selectively block the expression of PD-1 inhibitory signaling in CD8 T cells, B6 PD-1 - / - mice were used as donors of CD8 T cells in combination with CD8 T from wild-type B6 mice Cells are compared. Tumor diameter was measured periodically to detect anti-tumor function of PD-1 - / - and wild-type CD8 T cells. Results Our results showed that Rag-1 - / - recipient mice that did not receive CD8 T cell infusion continued to develop tumors. Recipient mice that received B6.PD-1 - / - CD8 T cell transfusions showed significantly shorter and faster tumors shrinking / disappearing significantly and faster than recipient mice receiving wild-type B6 CD8 T cell transfusions. Conclusion Blocking the PD-1 inhibitory signaling pathway in CD8 T cells can enhance the anti-tumor function of protective CD8 T cells and control and treat the occurrence and development of MAIDS-related tumors by retroviral infection.