本文报道一种准确、精密、简便、快速的超微量丙酮酸激酶连续监测法。PK对磷酸烯醇式丙酮酸的Km=0.068mmol/L指示酶LDH最适用量21.6单位,最适PH=7.5该反应在10秒钟之后至3分针呈良好的线性速率关系,批内CV％=3.14％,批间CV％=9.27巩,可测PK活力的线性范围以O至1450U/L.30例健康成人血清PK参考值为15—77U/L。PK对AMI珍断敏感性达100％,对肌营养不良、PK缺乏症的珍断具重要的临床意义。 This article reports an accurate, precise, simple and rapid ultra-trace pyruvate kinase continuous monitoring method. PK for phosphoenolpyruvate Km = 0.068 mmol / L Indicator LDH Optimal amount of 21.6 units, the optimal PH = 7.5 The reaction after 10 seconds to the third minute needle showed a good linear relationship between the rate of CV% = 3.14%, the inter-CV% = 9.27, the linear range of measurable PK activity from O to 1450U / L. 30 healthy adults serum PK reference value of 15-77U / L. The sensitivity of PK to AMI is up to 100%, which has important clinical significance for muscular dystrophy and PK deficiency.