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目的 高纤维素饮食对预防结肠癌有重要作用 ,但其具体作用环节与机制并不清楚。以人结肠癌Caco 2细胞为模型 ,研究 3种短链脂肪酸对细胞增殖、分化和转移的影响以及高纤维素饮食抗结肠癌的作用与意义。方法 以人高分化结肠癌Caco 2为模型 ,分别经细胞增殖能力对数转换法检测倍增时间、结肠癌上皮刷状缘细胞分化标志物组蛋白酶C生化检测及转移率测定 ,分析短链脂肪酸对人结肠癌细胞增殖、分化与转移的影响。结果 3种短链脂肪酸均可影响人结肠癌细胞的表型 ,显著延长肿瘤细胞倍增时间 ,增强癌细胞分化标志物组蛋白酶的表达 ,并明显抑制癌细胞的转移。结论 高纤维素饮食所含的短链脂肪酸通过抑制癌细胞的增殖、分化及转移而起到抗肿瘤作用。提高丁酸盐 /乙酸盐比率的纤维素饮食可增强其抗癌作用。
The purpose of high-fiber diet for the prevention of colon cancer has an important role, but its specific role and mechanism of mechanism is not clear. Using human colon cancer Caco 2 cells as a model, the effects of three kinds of short chain fatty acids on cell proliferation, differentiation and metastasis and the effect and significance of high-cellulose diet against colon cancer were studied. Methods Caco-2 human colon carcinoma was used as a model. The doubling time, logarithmic phase shift assay, histone biochemical detection and metastasis rate of brush border cells in colon carcinoma were determined respectively. The effect of short chain fatty acid Effects of proliferation, differentiation and metastasis of human colon cancer cells. Results All three kinds of short chain fatty acids could affect the phenotype of human colon cancer cells, significantly prolong the doubling time of tumor cells, enhance the expression of histone protein, a marker of cancer cell differentiation, and significantly inhibit the metastasis of cancer cells. Conclusion Short-chain fatty acids contained in high-fiber diet play an anti-tumor role by inhibiting the proliferation, differentiation and metastasis of cancer cells. A cellulose diet that increases the butyrate / acetate ratio may enhance its anticancer effect.