论文部分内容阅读
目的观察温脉通对血管紧张素Ⅱ(AngⅡ)诱导血管平滑肌细胞(VSMC)增殖的抑制作用及其对促VSMC生长因子的影响,以期探讨温脉通防治早期肢体动脉硬化性闭塞症(ASO)的作用机制。方法体外培养兔胸主动脉VSMC,以AngⅡ作为诱导因素,复制VSMC增殖模型,分别用温脉通全方及拆方药物血清进行干预。应用四唑盐(MTT)比色法测定各组VSMC增殖活性,用免疫细胞化学检测方法观察各组对血小板源性生长因子(PDGFBB)表达的影响。结果细胞经AngⅡ作用后其增殖活性明显增加,与空白组比较有显著性差异(P<0.01);而分别加入各组药物血清后,细胞增殖活性降低,与AngⅡ组比较有显著性差异(P<0.01),其中以大剂量组细胞增殖活性降低最为明显。血管平滑肌细胞PDGFBB免疫组化显色,空白组呈弱阳性表达(+/-);AngⅡ组呈强阳性表达(+++),与空白组比较有显著性差异;温脉通全方组PDGF-BB表达明显减弱(+),与空白组接近;温经益气拆方组和活血通脉拆方组PDGFBB呈中度表达(++),其阳性反应强度高于全方组,低于AngⅡ组。结论温脉通可呈剂量依赖性地抑制AngⅡ诱导的VSMC增殖、抑制PDGFBB的蛋白表达,提示温脉通防治ASO可能与抑制VSMC异常增殖及PDGF-BB的蛋白表达有关。
Objective To observe the effect of Wenmaitong on the proliferation of vascular smooth muscle cells (VSMC) induced by angiotensin II(AngII) and its effect on promoting VSMC growth factor, so as to investigate the effect of Wenmaitong on the prevention of early arteriosclerosis obliterans (ASO) The mechanism of action. Methods VSMCs of rabbit thoracic aorta were cultured in vitro. AngII was used as an inducing factor to replicate the proliferation model of VSMC. The rats were treated with Wenmaitongquanfang and split prescription drug serum respectively. The proliferative activity of VSMCs in each group was determined by tetrazolium (MTT) colorimetric method. The effect of each group on the expression of platelet-derived growth factor (PDGFBB) was observed by immunocytochemistry. Results The proliferative activity of cells after AngII treatment was significantly increased (P<0.01). Compared with the blank group, the proliferation activity of the cells was decreased, and there was a significant difference compared with the AngII group (P<0.01). <0.01), of which the cell proliferation activity in the high-dose group was the most obvious. The vascular smooth muscle cells PDGFBB immunohistochemical staining, the blank group showed weak positive expression (+/-); AngII group was strongly positive expression (+++), compared with the blank group was significantly different; Wenmai Tongquan group prescription PDGF The expression of -BB was significantly decreased (+) and was similar to that of blank group. PDGFBB in Wenjing Yiqi Decoction group and Huoxue Tongmai Decoction group was moderately expressed (++). The positive reaction intensity was higher than that of the whole group and lower than that of AngII group. . Conclusion Wenmaitong can inhibit the proliferation of AngII-induced VSMC and inhibit the protein expression of PDGFBB in a dose-dependent manner. It is suggested that Wenmaitong may be related to the inhibition of abnormal proliferation of VSMC and the expression of PDGF-BB protein.