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本文借用IL-2依赖细胞CTLL测定IL-2生物活性实验,检测了人外周血单个核细胞(PBM)在PHA促分裂剂的刺激下,上清内IL-2含量,并建立了一个微量的体外诱导人PBM产生IL-2的方法。实验结果示,体外诱导人PBM产生IL-2的最佳PHA浓度为1:250稀释,培养时间以44～48小时为宜。由于采用96孔板培养,诱生时需血量小。实验结果还示,诱导IL-2产生的PHA终浓度范围窄(1:250～1:500),而淋转相对宽(1:250～1:5000),单核细胞对IL-2的诱生似具有负调节作用,表现为去单核时诱生的IL-2活性增高。粗制的IL-2上清除能维持CTLL生长外,也能维持人PBM体外培养。
In this study, IL-2 bioactivity was measured by IL-2-dependent CTLL. IL-2 levels in the supernatant of human PBMC stimulated by PHA mitogen were measured and a trace amount of Methods to induce IL-2 production by human PBM in vitro. The experimental results show that the optimal concentration of PHA for inducing human PBM to produce IL-2 in vitro is 1: 250 dilution, and the culturing time is 44 to 48 hours. Due to the use of 96-well plate culture, induced blood when the need for small. The experimental results also showed that the final concentration range of PHA induced by IL-2 production was narrow (1: 250-1: 500) and the leaching was relatively wide (1: 250-1: 5000) Health seems to have a negative regulatory role, manifested as monocyte induced IL-2 activity increased. Crude IL-2 clearance can maintain CTLL growth, but also maintain human PBM in vitro.