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目的探讨丙戊酸钠对人喉癌Hep-2细胞的诱导分化作用。方法分别用1、2、3、4、5 mmol/L的丙戊酸钠孵育人喉癌Hep-2细胞,经过不同时间(12、24、36、48、72 h)孵育后,利用MTT方法检测人喉癌Hep-2细胞的增殖活性,利用RT-PCR方法检测3 mmol/L的丙戊酸钠处理人喉癌Hep-2细胞0、24、48、72 h后环氧化酶2(COX-2)和凋亡抑制蛋白Survivin m RNA的表达。结果丙戊酸钠对人喉癌Hep-2细胞生长分化具有明显的抑制作用,其作用表现为剂量依赖性(F值分别为10.621、21.196、32.945、42.864、60.236,P值均<0.01)和时间依赖性(F值分别为10.966、14.053、31.725、45.131、50.777,P值均<0.01);用3 mmol/L的丙戊酸钠处理人喉癌Hep-2细胞,检测发现随作用时间延长,COX-2和凋亡抑制蛋白Survivin m RNA的表达量均逐渐降低,差异有统计学意义(P<0.01)。结论丙戊酸钠对人喉癌Hep-2细胞的增殖分化具有明显的抑制作用,推测其作用机制为诱导COX-2和凋亡抑制蛋白Survivin m RNA的表达下调。
Objective To investigate the induction and differentiation of sodium valproate on human laryngeal carcinoma Hep-2 cells. Methods Human laryngeal carcinoma Hep-2 cells were incubated with sodium valproate at concentrations of 1, 2, 3, 4 and 5 mmol / L, respectively. After incubation at different times (12,24,36,48 and 72 h) The proliferation activity of human laryngeal carcinoma Hep-2 cells was detected by RT-PCR. The changes of cyclooxygenase 2 COX-2) and Survivin mRNA expression. Results Sodium valproate had a significant inhibitory effect on the growth and differentiation of human laryngeal carcinoma Hep-2 cells in a dose-dependent manner (F value 10.621,21.196,32.945,42.864,60.236, P <0.01) and (F = 10.966, 14.053, 31.725, 45.131 and 50.777, P <0.01 respectively). Hep-2 cells were treated with 3 mmol / L sodium valproate, and the results showed that with the prolongation of action time , COX-2 and Survivin m RNA expression decreased gradually, the difference was statistically significant (P <0.01). Conclusion Sodium valproate has obvious inhibitory effects on the proliferation and differentiation of human laryngeal carcinoma Hep-2 cells. The mechanism of action is that the expression of COX-2 and Survivin m RNA are down-regulated.