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该文介绍用单分子探测技术对液体中的染料分子R6G和R110进行高灵敏探测.以3σ为标准,达到的检测限分别为3.8*10-14MOL/L(R6G)和1*10-13MOL/L(R110),灵敏区内染料分子的平均数目<1.“,”This paper describes the ultrasensitive detection of R6G and R110 in a flowing aqueous sample,based on laser?induced fluorescence(LIF)combined with mode?locked pulsed excitation and time?correlated single?photon counting.A high repetition rate mode?locked argon ion laser operated at 514.5 nm was used as the excitation source.The laser beam was focused tightly into a thin wall capillary flow cell.The probe volume was about 12.6×10~-12?L.Fluorescence was collected at 90° using a ×40 microscope objective,and w...