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目的:探讨中药复方芪玉三龙汤对荷肺癌小鼠的抑瘤效应。方法:采用Lewis肺癌细胞株培养移植法建立肺癌荷瘤小鼠模型,随机分为模型组,化疗组,芪玉三龙汤高、中、低剂量组,联合组,每组8只;造模第2天开始芪玉三龙汤低、中、高剂量组小鼠分别按20.12、40.24、80.48g·kg~(-1)·d~(-1)灌胃给药,化疗组按照0.4mL顺铂腹腔注射给药,联合组以芪玉三龙汤高剂量灌胃和顺铂腹腔注射联合给药,模型组按照等量0.9%氯化钠溶液给药,1次/d,连续给药21d;称取瘤质量,计算抑瘤率;蛋白质免疫印迹法(Western blot)检测肿瘤组织Wnt1、Wnt2、Wnt5a蛋白水平。结果:与模型组比较,各用药组Wnt1、Wnt2、Wnt5a蛋白水平均显著降低(P<0.01);与化疗组比较,芪玉三龙汤中、高剂量组Wnt1、Wnt2、Wnt5a蛋白水平均显著降低(P<0.01)。芪玉三龙汤高剂量组、联合组生存状态优于化疗组;其高剂量抑制肿瘤生长作用较优,但量效关系不明显,其与顺铂联用有一定增效作用。芪玉三龙汤对肺癌移植瘤具有温和的抑制作用,其高剂量抑瘤效应较优。结论:芪玉三龙汤和化疗药联用具有协同效应,对肺肿瘤具有一定的抑制作用。
Objective: To investigate the antitumor effect of Chinese herbal compound Qiyu Sanlong Decoction on mice with lung cancer. Methods: Lewis lung cancer cell lines were cultured and transplanted to establish tumor-bearing mice model of lung cancer and were randomly divided into model group, chemotherapy group, high, medium and low dose of Qiyu Sanlong Decoction group, combined group, 8 rats in each group. On the second day, mice in low, medium and high doses of Qiyu Sanlong Decoction were intragastrically administrated by 20.12, 40.24 and 80.48 g · kg -1 · d -1 respectively, Cisplatin intraperitoneal injection, the combined group of high doses of stilbunal longan intragastric administration and cisplatin intraperitoneal injection, the model group according to the same amount of 0.9% sodium chloride solution, 1 / d, continuous administration The tumor weight was calculated and the tumor inhibition rate was calculated. The protein levels of Wnt1, Wnt2 and Wnt5a in tumor tissues were detected by Western blot. Results: Compared with the model group, the protein levels of Wnt1, Wnt2 and Wnt5a in the treated groups were significantly decreased (P <0.01). Compared with the chemotherapy group, the Wnt1, Wnt2 and Wnt5a protein levels in the medium and high doses of Qiyu San Long Decoction were significantly Decreased (P <0.01). The high-dose Qiyu Sanlong Decoction group showed better survival status than the chemotherapy group. The high-dose anti-tumor effect was better, but the dose-response relationship was not obvious. Combined with cisplatin, it had a certain synergistic effect. SJT has a mild inhibitory effect on lung cancer xenografts and its high-dose anti-tumor effect is better. Conclusion: The combination of Qiyu Sanlong Decoction and chemotherapeutic agents has a synergistic effect, which has a certain inhibitory effect on lung tumors.