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目的 研究昆明地区轮状病毒(RV)不同VP7血清型及NSP4基因变异与腹泻的流行及症状严重程度的关系。方法 对2002年和2003年分离于昆明地区的RV，用PCR分型法对四种主要的VP7血清型进行分型，并对从150份RV腹泻标本VP7血清型为G1、G3、G4型RV株中挑出的14份一般腹泻株和8份重症腹泻株的NSP4基因序列进行了分析，与来自GenBank Database的4株人RV(Wa、NUN、AU-1、Hochi)和3株动物RV(EW、OSU、SA11)以及中国不同地区流行株NSP4的基因变异情况进行了比较。结果 2002年昆明地区RV流行株以G1型为主，2003年RV腹泻株以G3型为主；昆明地区RV流行株间的氨基酸同源性高达98.9%～99.4%，22株流行株全都属于Wa组；NSP4变异与地域及VP7血清型无关；NSP4基因变异与RV腹泻临床症状严重程度不相关(P>0.05)。结论 不同年份相同季节不同地域流行的RV VP7血清型变异较大，而NSP4基因的相对保守性及其免疫原性使其有可能成为发展疫苗的候选基因。
Objective To investigate the prevalence and severity of symptoms of diarrhea in rotavirus (RV) serogroups and NSP4 in Kunming. Methods The RV serotypes isolated from Kunming in 2002 and 2003 were genotyped by polymerase chain reaction (PCR), and the serotypes of VP7 serotypes from 150 samples of RV diarrhea were classified as G1, G3 and G4 RV The sequences of NSP4 gene from 14 common diarrhea strains and 8 severe diarrhea strains isolated from the strains were analyzed and compared with 4 human RV (Wa, NUN, AU-1, Hochi) and 3 strains of RV EW, OSU, SA11) and the genetic variation of NSP4 in different parts of China. Results In 2002, the epidemic strains of RV in Kunming were mainly G1, and in 2003, the RV was mainly G3. The amino acid homology among RV strains in Kunming was as high as 98.9% -99.4%. All the 22 strains belonged to Wa Group; NSP4 mutation was not related to the region and VP7 serotypes; NSP4 gene mutation was not related to the severity of clinical symptoms of RV diarrhea (P> 0.05). Conclusion The variation of RV VP7 serotypes prevailing in different regions of the same season in different seasons is quite large. However, the relatively conservative and immunogenicity of NSP4 gene makes it possible to be a candidate gene for vaccine development.