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为了评价血清中葡萄糖-6-磷酸异构酶(glucose-6-phosphate isomerase,GPI)诊断类风湿关节炎(rheumatoidarthritis,RA)的价值,检索1990~2007年相关数据库,关于酶联免疫吸附法(enzyme linked immunosorbent assay,ELISA)检测血清中GPI诊断RA的诊断性试验文献,提取四格表资料,利用RevMan软件分析。15个研究对GPI检测异质性较大:χ2=191.65,P<0.00001,使用已知浓度血清作为标准品的5篇文献,具有同质性(χ2=6.97,P=0.14),合并OR=1.93(95%CI:1.40,2.67),有效性检验Z=4.00(P<0.0001)。15个研究合并诊断敏感性为38%,合并特异性为87%,曲线下面积为0.7846;5个使用相同标准品的研究合并诊断敏感性为25%,合并特异性为80%,曲线下面积为0.6279。GPI在RA诊断中的特异性较高而敏感性较差,可与高敏感性的其他标志物联合检测。
In order to evaluate the value of glucose-6-phosphate isomerase (GPI) in the diagnosis of rheumatoid arthritis (RA) in serum, the related databases from 1990 to 2007 were searched, and the relationship between enzyme-linked immunosorbent assay Enzyme linked immunosorbent assay (ELISA) was used to detect the diagnostic test of GPI in serum. The data of four grids were extracted and analyzed by RevMan software. Fifteen studies were more heterogeneous for GPI detection: χ2 = 191.65, P <0.00001. There were 5 articles with known concentrations of serum as standard (χ2 = 6.97, P = 0.14) 1.93 (95% CI: 1.40, 2.67), validity test Z = 4.00 (P <0.0001). The diagnostic sensitivity of the 15 studies was 38% with a combined specificity of 87% and an area under the curve of 0.7846. The five studies using the same standard had a combined diagnostic sensitivity of 25% and a pooled specificity of 80%. The area under the curve Is 0.6279. GPI in RA diagnosis of high specificity and less sensitive, with high sensitivity and other markers combined detection.