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试验研究了寒地粳稻（ＤＳ２＃）成熟胚为受体的ＪＱ７００型基因枪转化，寒地粳稻（ＤＳ３＃）下胚轴愈伤悬浮细胞与细胞团为受体的农杆菌ＬＢＡ４４０４共培养转化，以及寒地粳稻（ＤＳ２ＧＧ＃）未受精胚囊为受体的花粉管通道等转化途径的初步尝试。结果首先在基因枪转化途径上得到突破——成功地将ＮＰＴⅡ基因、ＧＵＳ基因及抗虫基因（Ｂ．ｔ）的ＤＮＡ片段导入寒地粳稻（ＤＳ２＃）成熟胚。以ＧＵＳ基因组织化学法检测外源基因的表达，结果转化水稻细胞的短暂表达为阳性。经过一系列组织培养过程，诱导获得了绿色转基因水稻植株，取其叶片进行外源基因的稳定遗传与表达检测，结果ＧＵＳ基因组织化学检测亦为阳性，Ｏｌｙｍｐｕｓ倒置显微镜镜检结果——转基因水稻（ＤＳ２＃）植株叶片的叶脉等维管组织中ＧＵＳ基因表达更强烈。本文只是对转化途径进行研究，关于Ｂ．ｔ基因的整合与表达的检测工作正在进行中
The JQ700-type particle bombardment was conducted to study the transformation of the JQ700-type gene gun from the mature embryos of cold-blooded japonica rice (DS2 #). The suspension cells of hypocotyls of cold-blooded japonica rice (DS3 #) were co-cultured with Agrobacterium tumefaciens LBA4404, (DS2GG #) unfertilized embryo sac as a receptor for pollen tube pathway and other transformation attempts. The results first broke through the path of gene gun transformation. The DNA fragments of NPTⅡ gene, GUS gene and B.t. were successfully introduced into the mature embryos of winter japonica rice (DS2 #). The expression of exogenous gene was detected by GUS histochemical method. As a result, transient expression of transformed rice cells was positive. After a series of tissue culture process, green transgenic rice plants were induced to obtain stable genetic and expression detection of exogenous genes. The results of GUS genomic chemistry were also positive. The results of Olympus inverted microscopy-transgenic rice DS2 #) plant leaves the veins and other vascular tissue GUS gene expression more strongly. This article is only a study of the conversion path, t gene integration and expression of the detection work is underway