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目的从富含多酚和多糖的狭叶红景天的茎和叶中提取出高质量RNA。方法比较了两种RNA提取方法,发现Trizal试剂法提取的RNA效果更好;同时,建立了一套完整检测RNA质量的科学方法。结果应用该方法从狭叶红景天植物茎提取的RNA的纯度是:A260/A280为2.098,A260/A230为1.138,浓度为214 ng/μl,电泳条带清晰,RNA完整性好。由此法所确定的高质量RNA可直接用于分子生物学实验。结论该方法可有效地从富含多酚和多糖的狭叶红景天的茎中提取高质量RNA,为高原珍稀药用植物分子生物学研究奠定基础。
Objective To extract high quality RNA from stems and leaves of Rhodiola crenulata rich in polyphenols and polysaccharides. Methods Two RNA extraction methods were compared and the RNA extracted by Trizal reagent method was found to be better. At the same time, a complete set of scientific methods for detecting RNA quality was established. Results The purity of RNA extracted from the stem of Rhodiola rosea plant by this method was 2.098 for A260 / A280, 1.138 for A260 / A230 and 214 ng / μl for A260 / A230. The bands of the bands were clear and the RNA integrity was good. The high quality RNA determined by this method can be directly used in molecular biology experiments. Conclusion The method can effectively extract high quality RNA from the stems of Rhodiola crenulata rich in polyphenols and polysaccharides, which lays the foundation for the molecular biology research of rare medicinal plants in plateau.