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本文报道应用新城鸡瘟病毒修饰过的自体肿瘤细胞疫苗对16例恶性脑瘤术后患者进行特异性主动免疫治疗,观察此瘤苗对患者细胞免疫功能的调整作用及瘤苗治疗后的全身反应和局部皮肤反应,探索瘤苗治疗恶性脑瘤的可行性。 将术中取下之自体肿瘤组织在无菌条件下以机械法制成单细胞悬液,按每100~(Hu)NDV(新城鸡瘟病毒)吸附4×10~6个活瘤细胞的比例以NDV感染瘤细胞,37℃、5％CO_2培养5小时,培养完毕以生理盐水洗涤3次,固定,灭能,调细胞浓度为1×10~7/ml,检菌无污染后备用。脑瘤术后患者从术后第一周开始,前臂皮内及皮下注射自体瘤苗1ml/次,左右前臂交替进行,每周一次,共4次。 免疫治疗前后分别采血检测患者细胞免疫功能,结果表明淋巴细胞转化率由治疗前的52.94±11.16％升至治疗后的60.88±11.78％,P<0.05;T细胞亚群OKT_4无明显变化,P<0.05;但OKT_8由29.06±9.84％
This article reports the use of Newcastle disease virus modified autologous tumor cell vaccine on 16 cases of malignant brain tumor patients after specific active immunotherapy to observe the effects of this vaccine on the cellular immune function in patients with tumor therapy and systemic response and Local skin reaction to explore the feasibility of tumor vaccine treatment of malignant brain tumors. The autologous tumor tissue removed during operation was made into a single cell suspension by mechanical method under the condition of aseptic conditions, and the ratio of 4 × 10 ~ 6 viable cells per 100 ~ (Hu) NDV (Newcastle disease virus) Infected tumor cells were incubated at 37 ° C for 5 hours in 5% CO 2. After the culture was completed, the cells were washed with normal saline for 3 times. The cells were fixed and killed, and the cell concentration was adjusted to 1 × 10 -7 / ml. Patients with brain tumor surgery from the first week after surgery, forearm intradermal and subcutaneous injection of autologous tumor vaccine 1ml / time, left and right forearm alternation, once a week, a total of 4 times. Blood samples were collected before and after immunotherapy to detect cellular immune function. The results showed that the rate of lymphocyte transformation increased from 52.94 ± 11.16% before treatment to 60.88 ± 11.78% after treatment, P <0.05. There was no significant change in T cell subsets OKT_4, P < 0.05; but OKT_8 increased from 29.06 ± 9.84%