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目的:比较人参皂苷Rh2、榄香烯与阿霉素对胶质瘤细胞的体外抑制及凋亡诱导作用,以及药物杀伤的时效关系。方法:体外培养胶质瘤细胞,用MTT肿瘤体外药敏法比较人参皂苷Rh2、榄香烯与阿霉素对胶质瘤细胞系C6、SHg-44、U-251的抑制作用,并绘制细胞存活率-时间曲线。用流式细胞术检测3种药物作用后细胞凋亡情况。结果:3种药物对胶质瘤细胞系C6、SHg-44、U-251的抑制作用呈明显的剂量依赖性,随浓度的增加而增强。人参皂苷Rh2对胶质瘤细胞系的抑制作用明显高于榄香烯和阿霉素,与后两者比较差异有显著性(P<0.01)。3种药物对胶质瘤细胞的抑制作用均呈时间依赖关系,随时间延长而增强,且低浓度的人参皂苷Rh2短时间(12 h)就有生长抑制作用,而榄香烯和阿霉素的起效时间较缓慢。3种肿瘤细胞经低浓度的3种药物作用后均可出现凋亡,其中人参皂苷Rh2凋亡细胞比率明显高于其他两组,差异有显著性(P<0.01)。结论:人参皂苷Rh2对胶质瘤具有较好的生长抑制和凋亡诱导作用,起效时间早于榄香烯和阿霉素。人参皂苷Rh2良好的抑瘤效果和迅速的起效时间具有应用于胶质瘤治疗的潜在价值。
OBJECTIVE: To compare the inhibitory and apoptosis-inducing effects of ginsenoside Rh2, elemene and doxorubicin on glioma cells in vitro and the time-effect relationship of drug killing. METHODS: Glioma cells were cultured in vitro and the inhibitory effects of ginsenoside Rh2, elemene and doxorubicin on glioma cell lines C6, SHg-44, and U-251 were compared using MTT tumor in vitro susceptibility assay. Survival-time curve. Flow cytometry was used to detect the apoptosis of the three drugs. RESULTS: The inhibitory effects of the three drugs on glioma cell lines C6, SHg-44 and U-251 were dose-dependent and increased with increasing concentrations. The inhibitory effect of ginsenoside Rh2 on glioma cell lines was significantly higher than that of elemene and adriamycin, which was significantly different from the latter two groups (P<0.01). The inhibitory effects of the three drugs on glioma cells were time-dependent and increased with time. Low concentrations of ginsenoside Rh2 had growth inhibitory effects for a short period of time (12 h), while elemene and doxorubicin The onset time is relatively slow. The apoptosis of three kinds of tumor cells could be observed after treatment with low concentration of three drugs, among which the ratio of apoptotic cells of ginsenoside Rh2 was significantly higher than that of the other two groups (P<0.01). Conclusion: Ginsenoside Rh2 has good growth inhibition and apoptosis-inducing effect on gliomas, and its onset time is earlier than that of elemene and adriamycin. Ginsenoside Rh2’s good anti-tumor effect and rapid onset time have potential value in the treatment of gliomas.