论文部分内容阅读
以人主动脉平滑肌细胞（ＳＭＣ）为模型，用３Ｈ－ＴｄＲ掺入量测定法观察了降钙素基因相关肽（ＣＧＲＰ）对细胞增殖的影响，并对细胞内Ｃａ２＋浓度进行了测定。结果表明：ＣＧＲＰ对人主动脉ＳＭＣ有明显抑制作用，呈浓度依赖性，最佳浓度为１０ｎｍｏｌ／Ｌ，抑制率达４７．４４％。胞浆内游离Ｃａ２＋浓度明显降低，提示ＣＧＲＰ降低细胞内Ｃａ２＋浓度可能是其抑制ＳＭＣ增殖的作用机制之一。
The effects of calcitonin gene-related peptide (CGRP) on cell proliferation were observed by 3H-TdR incorporation assay using human aortic smooth muscle cells (SMC) as a model, and the intracellular Ca2 + concentration was measured. The results showed that CGRP had a significant inhibitory effect on human aortic SMC in a concentration-dependent manner. The optimal concentration was 10 nmol / L and the inhibitory rate was 47.44%. Cytoplasmic free Ca2 + concentration was significantly reduced, suggesting that CGRP reduce intracellular Ca2 concentration may be one of its mechanisms of inhibition of SMC proliferation.