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目的:检测趋化因子受体CXCR7(chemokine receptor 7)及其配体在子宫内膜癌组织和子宫内膜癌细胞中的表达,并研究雌激素对该受体的调控作用。方法:首先通过RT-PCR分析20种趋化因子受体在子宫内膜癌细胞株Ishikawa(ERα阳性)和AN3CA(ERα阴性)的转录水平,筛选出子宫内膜癌细胞株高表达的趋化因子受体,以免疫细胞化学染色法测定该受体及其相应配体在子宫内膜癌组织和细胞中的表达,并用蛋白质印迹法观察雌激素对该受体蛋白水平表达的影响。结果:(1)CXCR7 mRNA在子宫内膜癌细胞株Ishikawa、AN3CA均呈高表达,免疫细胞化学示子宫内膜癌组织和Ishikawa、AN3CA细胞表达CXCR7及其配体SDF-1,不表达I-TAC;(2)在Ishikawa中,雌激素在一定浓度范围内上调细胞中CXCR7蛋白水平的表达,而在AN3CA中,雌激素对CXCR7蛋白并无明显的调节作用。结论:CXCR7/SDF-1高表达于子宫内膜癌组织和子宫内膜癌细胞中,且雌激素上调CXCR7的表达,表明CXCR7/SDF-1可能在子宫内膜癌中发挥调控作用。
OBJECTIVE: To detect the expression of chemokine receptor 7 (CXCR7) and its ligand in endometrial carcinoma and endometrial carcinoma cells and to study the regulatory effect of estrogen on this receptor. Methods: The transcription level of 20 chemokine receptors in endometrial carcinoma cell lines Ishikawa (ERα-positive) and AN3CA (ERα-negative) was analyzed by RT-PCR, and chemotaxis of highly expressed endometrial cancer cell lines was screened Factor receptor was detected by immunocytochemical staining of the receptor and its corresponding ligand in endometrial cancer tissues and cells, and Western blotting was used to observe the effect of estrogen on the expression of the receptor protein. Results: (1) CXCR7 mRNA was highly expressed in Ishikawa and AN3CA cell lines. Immunocytochemistry showed that endometrial carcinoma and Ishikawa, AN3CA cells expressed CXCR7 and its ligand SDF-1, but not I- TAC; (2) In Ishikawa, estrogen upregulated the expression of CXCR7 protein in a certain concentration range, while in AN3CA, estrogen had no obvious regulation on CXCR7 protein. Conclusion: CXCR7 / SDF-1 is overexpressed in endometrial carcinoma and endometrial cancer cells, and estrogen up-regulates the expression of CXCR7, indicating that CXCR7 / SDF-1 may play a regulatory role in endometrial cancer.