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通过挖掘大豆基因组信息并参考拟南芥同源基因序列,分析了大豆(Glycine max)的生长素响应因子GmARF16和Gm-miR160作用位点,设计出mGmARF16序列并成功构建了pGmARF16∷mGmARF16双元表达载体。此外,通过实时荧光定量PCR(qPCR)分析了GmARF16表达模式,发现该基因在大豆根、茎、叶、花、果荚和种子中均有一定量的表达,其中花中表达最强,茎中表达最弱;复叶中表达量高于子叶和第一对真叶。
By digging soybean genome information and referring to Arabidopsis thaliana homologous gene sequences, the auxin responsive sites GmARF16 and Gm-miR160 of soybean (Glycine max) were analyzed. The mGmARF16 sequence was designed and the dual expression of pGmARF16 :: mGmARF16 Vector. In addition, the expression pattern of GmARF16 was analyzed by real-time quantitative PCR (qPCR) and found that the gene was expressed in roots, stems, leaves, flowers, pods and seeds in a certain amount, of which the expression was the strongest in flowers and the expression in stems The weakest; the expression level in the compound leaf is higher than that of the cotyledon and the first pair of true leaves.