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目的:评价奥林巴斯AU2700全自动生化分析仪测定电解质项目的分析性能。方法:按照美国临床实验室标准化组织(CLSI)指南文件和其他相关文献的实验方案,分析奥林巴斯AU2700全自动生化分析仪测定电解质项目的精密度、准确度、线性及可报告范围等。结果:AU2700全自动生化分析仪检测K+批内CV分别为1.84%、0.89%,批间CV分别为2.08%、4.20%,Na+批内CV分别为0.05%、0.94%,Na+批间CV分别0.64%、0.81%,Cl-批内CV分别为0.88%、0.75%,Cl-批间CV分别为1.12%、1.21%,均满足CLIA88标准。正确度:检测结果在室间质评的允许范围。线性:K+Y=1.0085X-0.0312,a=1.0085,r 2=0.99;Na+Y=9 998X-0.0055,a=0.9998,r 2=1;Cl-Y=0.9895X+1.8413,a=0.9895,r 2=0.99。可报告范围:K+0.68~11.8mmol/L,Na+37~288.6mmol/L,Cl-37.1~246.6mmol/L。结论:奥林巴斯AU2700全自动生化分析仪检测电解质项目在精密度、准确度、线性范围等方面均达到了生物化学检验实验室的要求,可用于临床标本检测。
OBJECTIVE: To evaluate the analytical performance of Olympus AU2700 automatic biochemical analyzer in the determination of electrolyte projects. Methods: The precision, accuracy, linearity and reportable range of the electrolyte project of Olympus AU2700 automatic biochemical analyzer were analyzed in accordance with the guidelines of the American Society of Clinical Laboratory Standards (CLSI) and other related literatures. Results: The K + intra-assay CV of the AU2700 automatic biochemical analyzer were 1.84% and 0.89% respectively. The inter-assay CV was 2.08% and 4.20% respectively. The intra-assay CV was 0.05% and 0.94% respectively. % And 0.81% respectively. The CVs of Cl-batch were 0.88% and 0.75% respectively, and the inter-Cl CVs were 1.12% and 1.21% respectively, which all met the CLIA88 standard. Accuracy: test results in the room quality assessment of the allowable range. A = 0.9998, r 2 = 1; Cl-Y = 0.9895X + 1.8413, a = 0.9895 , r 2 = 0.99. Reportable range: K + 0.68 ~ 11.8mmol / L, Na + 37 ~ 288.6mmol / L, Cl-37.1 ~ 246.6mmol / L. Conclusion: Olympus AU2700 automatic biochemical analyzer detection of electrolyte projects in the precision, accuracy, linear range, etc. have reached the requirements of biochemical laboratory, clinical specimens can be used for testing.