运用改良Ha(?)tchen标本性染色质(Barr小体)检测法,观察雌雄配对同种异体经冷冻处理后的动脉移植段血管内皮细胞的形态变化及修复过程.结果表明,修复后新生的内皮细胞来源于受体;两步冷冻(-60℃和-196℃)法处理异体动脉移植段,其内皮完整,抗原性减弱,移植排斥反尖减缓;内皮脱落速度变慢,修复加快,术后12周通畅率达87.5%;改良的Ha(?)tchen标本Barr小体检测法具有操作简便,经济实用,结果具有重复性好等优点. The morphological changes and repair process of vascular endothelial cells after arteriovenous grafting after male and female mating were observed by using modified Hachen? Tchen sample (Barr body) assay. The results showed that the repair of neonate Endothelial cells derived from the recipient; two-step freezing (-60 ℃ and -196 ℃) method of allogeneic artery graft, the integrity of the endothelium, reduced antigenicity, anti-rejection graft slowed down; endothelial excretion slowed down, repair faster, The patency rate was 87.5% after 12 weeks. The improved Barr body detection method of Hachen? Tchen specimens was simple, economical and practical, with good reproducibility.