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用两种方法制备生物素ｃ－ｍｙｃ癌基因探针，通过ＲＮＡ－ＤＮＡ斑点杂交技术，检测了白血病３０例和２种白血病细胞株中ｃ－ｍｙｃ原癌基因的ＲＮＡ表达水平。结果显示，白血病细胞株、急性白血病和慢性粒细胞性白血病急性变患者的ｃ－ｍｙｃ的ＲＮＡ表达水平明显高于正常人和患其它血液病的患者，提示ｃ－ｍｙｃＲＮＡ水平的判定可提供一些有意义的临床信息；同时探讨了生物素和光敏生物素标记核酸探针的优越性。
The biotin c-myc oncogene probes were prepared by two methods. The RNA expression levels of c-myc oncogene in 30 leukemia and 2 leukemia cell lines were detected by RNA-DNA dot blot hybridization. The results showed that leukemia cell lines, acute leukemia and acute myeloid leukemia in patients with c-myc RNA expression was significantly higher than normal and other blood diseases in patients, suggesting that the c-mycRNA level of the decision can provide some have Significance of the clinical information; also discussed the advantages of biotin and biotin-labeled nucleic acid probe.