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目的:探讨复方黄栌口服液对小鼠黄疸型肝炎模型肝脏指标的影响。方法:将72只KM小鼠随机分为空白组,模型组,护肝宁片组(1.012 5 g/kg),大、中、小剂量复方黄栌口服液组(30、15、7.5 mL/kg),每组12只,空白组和模型组灌胃相同体积的生理盐水,连续灌胃相应的药物10 d。各组于实验第8天,灌胃ANIT造模,48 h后取材。小鼠脱颈椎处死后,从肝右叶切取肝组织1块,10%中性福尔马林固定,脱水,包埋,切片,HE染色,观察组织病理学变化。另取肝脏,用冷生理盐水制匀浆,检测肝匀浆MDA、SOD、TC、TG。结果:与模型组比,大、中、小剂量复方黄栌口服液组均可显著降低肝脏TC、TG、MDA水平(P<0.01),显著升高肝脏SOD水平(P<0.01)。大、小剂量复方黄栌口服液组可显著降低小鼠肝指数(P<0.01),中剂量复方黄栌口服液组可明显降低小鼠肝指数(P<0.05)。结论:复方黄栌口服液组和护肝宁片组可明显减轻造模所致的小鼠肝损伤。
Objective: To investigate the effect of Fufang Huangjian Oral Liquid on liver indexes of mice with jaundice hepatitis model. Methods: Seventy two KM mice were randomly divided into blank group, model group and Huganing tablet group (1.012 5 g / kg). The large, medium and small doses of compound Huangji Oral Liquid group (30,15,7.5 mL / kg ), 12 rats in each group. The blank group and the model group were given the same volume of normal saline, and the corresponding drugs were given continuously for 10 days. The experimental group on the 8th day, intragastric ANIT modeling, 48 h after the material. After the mice were sacrificed and the cervical vertebrae were sacrificed, one piece of liver tissue was obtained from the right lobe of the liver, fixed in 10% neutral formalin, dehydrated, embedded, sectioned and stained with HE for observation of histopathological changes. Another liver, homogenate with cold saline, liver homogenate MDA, SOD, TC, TG. Results: Compared with the model group, the contents of TC, TG and MDA in the liver of the large, medium and small doses of compound Huangji Oral Liquid group were significantly decreased (P <0.01) and significantly increased in the liver (P <0.01). Large and small doses of Huang Huang oral liquid group can significantly reduce the liver index (P <0.01), medium dose Huang Huang oral liquid group can significantly reduce the liver index (P <0.05). Conclusion: The compound Huangpi oral liquid group and Huganing tablet group can obviously reduce the liver damage in mice induced by modeling.