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目的了解广东省珠江三角洲地区吸毒者HIV-1亚型的流行规律及其与国际参考株的同源性。方法应用套式聚合酶链反应(PCR)对43例采自广东省珠江三角洲HIV-1抗体阳性的吸毒者淋巴细胞富集液,并对其核酸样品进行扩增,使用ABI377型测序仪对扩增产物测序后,对其ENV基因C2-V3段的核酸序列进行比较分析。结果43份血样中,29份为07-BC重组亚型,与国际参考株CN.97.C54A最近,基因离散率为(2.291±1.055)%,组内离散率为(3.534±1.306)%;9份为AE重组亚型,与国际参考株01AE.TH.90.CM240最近,基因离散率为(8.068±0.534)%,组内离散率为(2.823±1.235)%;3份为08-BC重组亚型,与国际参考株97CNGX-9F最近,基因离散率为(1.403±0.681)%,组内离散率为(1.507±0.681)%;2份为泰国B(B′)亚型,与国际参考株RL42最近,基因离散率为(7.335±3.330)%,组内距离为8.52%。结论广东省珠江三角洲地区吸毒者感染的HIV-1以曾经主要在西北地区吸毒人群中流行的07-BC重组亚型为主,也存在主要以性传播途径感染的人群中流行的AE重组亚型,提示珠江三角洲地区吸毒者中流行的HIV-1亚型趋于多样化。
Objective To understand the prevalence of HIV-1 subtype of drug addicts in the Pearl River Delta of Guangdong Province and its homology with the international reference strains. Methods 43 cases of HIV-1 antibody-positive lymphocyte enrichment fluid collected from the Pearl River Delta in Guangdong Province were amplified by polymerase chain reaction (PCR), and their nucleic acid samples were amplified. Using ABI377 sequencer, After sequencing the amplified product, the nucleotide sequences of the C2-V3 of the ENV gene were compared. RESULTS: Of the 43 blood samples, 29 were of the 07-BC recombinant subtype. The most recent genetic variation was (2.291 ± 1.055)% with the international reference strain CN.97.C54A. The in-group discrete rate was 3.534 ± 1.306% Nine were AE subtypes, with the most recent genetic variation of (8.068 ± 0.534)%, with the international reference strain 01AE.TH.90.CM240, the intra-group divergence rate was (2.823 ± 1.235)%; (1.403 ± 0.681)%, the intra-group discrete rate was (1.507 ± 0.681)%; 2 copies were B (B ’) subtype of Thailand, and the international sub-type 97CNGX- Reference strain RL42 recently, the gene dispersion rate was (7.335 ± 3.330)%, intra-group distance of 8.52%. Conclusions HIV-1 infected with drug addicts in the Pearl River Delta Region of Guangdong Province is dominated by the 07-BC recombinant subtype that was once predominant among drug users in the northwestern region. There are also AE subtypes that are prevalent among sexually transmitted infections , Suggesting that the prevalence of HIV-1 subtypes among drug abusers in the Pearl River Delta tends to be diversified.