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目的：探讨内皮素－１（ＥＴ－１）、内皮素Ａ型受体（ＥＴＡＲ）及肿瘤坏死因子α（ＴＮＦα）在烧冲复合伤病理生理过程中的作用和地位。方法：应用放射免疫及原位杂交和斑点杂交等核酸分子生物学技术，观测大鼠烧伤和烧冲复合伤后肺组织ＥＴ－１、ＥＴＲ及ＴＮＦα的转录表达变化、相互关系及与组织细胞损伤的关系。结果：伤后早期肺组织ＴＮＦα及ＥＴ－１的ｍＲＮＡ转录及其产生分泌均显著增加，血浆一氧化氮（ＮＯ）也有所增加，肺组织中的内皮素Ａ型受体（ＥＴＡＲ）ｍＲＮＡ升高，但ＥＴＲ却“下调”，各项指标变化均以烧冲复合伤组为显著，并与肺组织病理变化相关。结论：ＥＴ－１及其受体ＥＴＡＲ等在烧冲复合伤后肺的病理生理过程中可能起着重要作用。
Objective: To investigate the role and status of endothelin-1 (ET-1), endothelin A receptor (ETAR) and tumor necrosis factor α (TNFα) in the pathophysiology of burn-red combined injury. Methods: The expression of ET-1, ETR and TNFα in lung tissues were detected by radioimmunoassay, in situ hybridization and dot blot hybridization. The relationship between the expression of ET-1, ETR and TNFα, Relationship. Results: The mRNA and protein levels of TNFα and ET-1 in the early stage of post-traumatic injury were significantly increased, and the level of plasma nitric oxide (NO) was also increased. The level of endothelin A receptor (ETAR) mRNA in lung tissue was increased , But the ETR was “down”. The changes of the indexes were all significant in the burn-burn combined injury group and were related to the pathological changes of the lung tissue. CONCLUSION: ET-1 and its receptor ETAR may play an important role in the pathophysiology of lung after burn-red combined injury.