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采用He-Ne激光辐照(5 mW/mm2)和增强紫外线B(UV-B)辐射处理“晋麦8号”小麦,利用聚丙烯酰胺凝胶电泳(PAGE)技术分析UV-B和He-Ne激光辐照后小麦叶片的过氧化物(POD)同工酶、过氧化氢(CAT)同工酶、酯酶(EST)同工酶、三磷酸腺苷酶(ATPase)同工酶、苹果酸脱氢酶(MDH)同工酶各种酶谱的变化情况。PAGE结果显示,小麦叶片经UV-B辐照后诱导出2条新POD同工酶谱带,但明显降低了总的POD活性,对CAT,EST,ATPase和MDH同工酶酶谱数目影响不大,但酶带强度减弱,同工酶活性明显下降,说明UV-B辐射抑制了植物的基因表达;He-Ne激光处理可促进同工酶的基因表达,使酶带增强;UV-B辐射后再用He-Ne激光处理,激活了UV-B辐射钝化的基因,促进了同工酶的基因表达,使酶活性增强。研究发现,一定剂量的He-Ne激光辐照可以通过促进小麦叶片中同工酶的基因表达来增强植物的抗逆性。
Wheat “Jinmai No.8” was treated with He-Ne laser irradiation (5 mW / mm2) and enhanced ultraviolet B (UV-B) radiation and analyzed by polyacrylamide gel electrophoresis Peroxidase (POD), hydrogen peroxide (CAT) isozyme, esterase (EST) isoenzyme, ATPase isoenzyme, malic acid Dehydrogenase (MDH) isozymes various zymogram changes. PAGE results showed that two new POD isoenzymes bands were induced in wheat leaves after UV-B irradiation, but the total POD activity was significantly reduced, but the number of CAT, EST, ATPase and MDH isozymes did not affect But the intensity of enzyme band decreased and the isoenzyme activity decreased obviously, indicating that UV-B radiation inhibited the gene expression of plants; He-Ne laser treatment could promote the gene expression of isoenzyme and enhance the enzyme band; UV-B radiation He-Ne laser treatment followed by activation of UV-B radiation passivation of genes, promote the gene expression of isozymes, Enzyme activity. The study found that a certain dose of He-Ne laser irradiation can enhance plant stress resistance by promoting the gene expression of isozymes in wheat leaves.