论文部分内容阅读
本文旨在探讨大鼠蛛网膜下腔出血(SAH)诱发脑血管痉挛时脑底动脉降钙素基因相关肽(CGRP)能神经纤维的变化。大鼠随机分为正常组、蛛网膜下腔出血组。SAH组大鼠取股动脉自体血注入蛛网膜下腔,正常组动物不做任何处理。3d后灌注固定后,应用免疫组织化学ABC法,对两组大鼠脑底动脉CGRP能神经纤维变化进行观察。结果显示:正常组大鼠脑底动脉可见棕褐色,细线状CGRP能免疫反应阳性纤维;而SAH后3d组大鼠脑底动脉与正常组大鼠脑底动脉相比CGRP能神经纤维密度明显减少,具有统计学意义。以上结果提示脑底动脉CGRP能神经纤维在蛛网膜下腔出血诱发脑血管痉挛的病理生理过程中可能发挥重要的作用。
This article aims to investigate the changes of calcitonin gene-related peptide (CGRP) nerve fibers in cerebral arteries in rats with subarachnoid hemorrhage (SAH) -induced cerebral vasospasm. Rats were randomly divided into normal group, subarachnoid hemorrhage group. In SAH group, autologous blood from the femoral artery was injected into the subarachnoid space. Normal animals were not treated. Three days after perfusion fixation, immunohistochemical ABC method was used to observe the change of CGRP nerve fibers in the cerebral arteries of the two groups. The results showed that the CGRP-positive fibers were visible in the basilar artery of rats in normal group. The density of CGRP nerve fibers in cerebral artery of rats in 3d group after SAH was significantly higher than that in normal group Reduce, with statistical significance. The above results suggest that CGRP nerve fibers in the cerebral artery may play an important role in the pathophysiology of cerebral vasospasm induced by subarachnoid hemorrhage.