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目的测定优可力片中人参皂苷Rg1、Re和Rb1含量的方法。方法采用反相高效液相色谱法。色谱柱:Kromasil C18(250 mm×4.6 mm,5μm),流动相:乙腈-体积分数为0.1%的磷酸水溶液(梯度洗脱),流速:1.0 mL.min-1,检测波长:203 nm,柱温:30℃,进样量:10μL。结果人参皂苷Rg1、Re及Rb1的检测质量浓度分别在14.1～282.0(r=0.999 6)、22.0～440.0(r=0.999 9)、10.2～204.0 mg.L-1(r=0.999 9)内与各自峰面积呈良好线性关系;三者的平均回收率分别为98.3%、99.7%和98.7%,RSD(%,n=9)分别为1.0、0.9和1.6。结论该方法可用于优可力片的质量控制。
Objective To determine the content of ginsenosides Rg1, Re and Rb1 in Youkuel tablets. Methods Using reversed-phase high performance liquid chromatography. Column: Kromasil C18 (250 mm × 4.6 mm, 5μm), mobile phase: acetonitrile-0.1% phosphoric acid aqueous solution (gradient elution), flow rate: 1.0 mL · min-1, detection wavelength: 203 nm, Temperature: 30 ℃, injection volume: 10μL. Results The detection concentrations of ginsenosides Rg1, Re and Rb1 were in the range of 14.1-2828 (r = 0.999 6), 22.0-440.0 (r = 0.999 9), 10.2-204.0 mg.L-1 (r = 0.999 9) The average recoveries of the three were 98.3%, 99.7% and 98.7%, respectively. The RSDs (%, n = 9) were 1.0, 0.9 and 1.6, respectively. Conclusion This method can be used for the quality control of Youkueli tablets.