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目的 探讨地塞米松产前干预是否对宫内窘迫胎鼠脑损害有保护作用 ,以寻找围产期脑损害早期治疗的新途径。 方法 将 57只胎龄为 2 0 d的 SD大鼠的胎鼠随机分为 5组 ,即假手术组 (对照组 ,C组 ) 1 1只 ;宫内窘迫模型作为窘迫对照组 ( E组 ) 1 0只 ;宫内窘迫模型并在钳夹血管前、钳夹同时以及再灌注同时对母鼠静脉注射地塞米松 ( 1 mg/ kg)者分别为治疗 I组 ( D1组 ) 1 5只 , 组( D2 组 ) 1 2只和 组 ( D3 组 ) 9只。在再灌注 3 0 min后取脑检测各组胎鼠脑细胞内外钙、钠、钾含量。 结果 ( 1 ) E、D1、D2 和 D3 组胎鼠脑细胞内游离钙离子浓度分别为 ( 552± 94 )、( 43 8± 1 0 5)、( 44 5± 57)和 ( 456± 1 1 0 ) nmol/ L,均明显高于对照组 ( 3 1 5± 87) nmol/ L ( P<0 .0 1 )。 ( 2 ) D1组、D2 组、D3 组对母鼠注射地塞米松 ,其胎鼠脑细胞内游离钙离子浓度仍高于 C组 ,但亦均明显低于 E组 ( P<0 .0 1或0 .0 5)。( 3 ) D3 组脑组织总钠含量 ( 485± 1 85) mmol/ kg干脑 ,明显低于 E组 ( 64 1± 1 55) mmol/ kg干脑 ( P<0 .0 5)。 结论 应用地塞米松后 ,胎鼠脑细胞钙、钠超载明显减轻 ,提示产前注射地塞米松可能对胎鼠宫内窘迫脑损害有一定的保护作用。
Objective To investigate whether prenatal intervention with dexamethasone can protect brain injury induced by intrauterine fetal distress in order to find a new way of early treatment of perinatal brain damage. Methods Fetal rats of 57 SD rats with gestational age of 20 days were randomly divided into five groups, namely, sham operation group (control group, C group) 11 rats; intrauterine distress model as distress control group (E group) 10; intrauterine distress model and in the clamp before and after the clamp, as well as reperfusion while intravenous injection of dexamethasone (1 mg / kg) in the females were treated group I (D1 group) 15, There were 12 mice in group (D2) and 9 mice in group (D3). At 30 minutes after reperfusion, brain contents of calcium, sodium and potassium in fetal rat brain were detected by computer. Results (1) The intracellular free calcium concentration in fetal rat brain was (552 ± 94), (438 ± 105), (445 ± 57) and (456 ± 1 1 0) nmol / L were significantly higher than that of the control group (31.5 ± 87) nmol / L (P <0.01). (2) In group D1, group D2 and group D3, dexamethasone was injected into female rats, and the intracellular free calcium concentration in fetal rats was still higher than that in group C, but also significantly lower than that in group E (P <0.01) Or 0 .0 5). (3) The total sodium content in brain tissue of D3 group was (485 ± 1 85) mmol / kg dry brain, which was significantly lower than that of group E (64 1 ± 1 55) mmol / kg dry brain (P <0.05). Conclusion Dexamethasone significantly reduced the calcium and sodium overload in fetal rat brain cells, suggesting that prenatal injection of dexamethasone may have some protective effect on fetal rat intrauterine fetal distress brain damage.