论文部分内容阅读
目的研究人类白细胞抗原G(human leukocyte antigen,HLA-G)及其异构体mRNA在人类着床前胚胎的表达,探讨HLA-G在人类胚胎早期发育中的作用和意义。方法第四军医大学唐都医院妇产科生殖医学中心2003-01-2005-12期间,以体外受精-胚胎移植技术助孕过程中剩余的患者自愿捐赠的人类早期胚胎作为研究对象,采用巢式RT-PCR检测早期胚胎HLA-G及其异构体mRNA的表达。结果检测20个受精后2~3d的人类卵裂期胚胎,2、4、6、8细胞期胚胎各5个,有7个胚胎表达HLA-G及其部分的异构体mRNA。检测5个受精后4d的人类桑葚胚,全部表达HLA-G及其部分的异构体mRNA。检测25个受精后6d的扩张期囊胚,全部受检囊胚均表达HLA-G mRNA,但异构体表达不同。在受检的25个扩张期囊胚中,20个表达HLA-G1(表达率80·0%),4个表达G2(表达率16·0%),25个表达G3(表达率100%)、24个表达G4(表达率96·0%),5个表达G5(表达率20·0%),8个表达G6(表达率32·0%)。结论着床前的人类胚胎表达HLA-G及其异构体mRNA,其阳性表达胚胎的比例,随着胚胎发育的进程而增加。HLA-G异构体在人类着床前胚胎差异表达。
Objective To study the expression of human leukocyte antigen (HLA-G) and its isoform mRNA in human preimplantation embryos and to explore the role and significance of HLA-G in the early development of human embryos. Methods During the period of 2003-01-2005-12, the embryos of human donors voluntarily donated by the remaining pregnant women assisted by in vitro fertilization and embryo transfer were selected as research objects. The nested The mRNA expression of HLA-G and its isoforms in early embryos was detected by RT-PCR. Results 20 human embryos of 2 ~ 3d after fertilization, 5 embryos of 2, 4, 6 and 8 were detected. Seven embryos expressed HLA-G and its part of isoforms mRNA. Five human morulae were detected 4 days after fertilization, all of which expressed isoforms mRNA of HLA-G and its part. After 25 days of fertilization, the expanded blastocysts were detected, and all the examined blastulas expressed HLA-G mRNA, but the isoforms were different. Of the 25 expanded blastocysts examined, 20 expressed HLA-G1 (80.0%), 4 expressed G2 (16.0%), and 25 expressed G3 (100% expressed) , 24 expressed G4 (96.0%), 5 expressed G5 (20.0%) and 8 expressed G6 (32.0%). Conclusion The pre-implantation human embryos expressed HLA-G and its isoforms mRNA, the proportion of positive expressing embryos increased with the progress of embryo development. HLA-G isoforms differentially expressed in human preimplantation embryos.