【摘 要】
：
Human cyclophilin 33 (hCyP33), found in 1996, consists of an RNA-binding domain in N-terminus, a cyclophilin domain in C-terminus and a connected part between t
【机 构】
：
State Key Laboratory of Functional Polymer Materials for Adsorption and Separation
【基金项目】
：
国家重点基础研究发展计划(973计划);教育部科学技术研究项目;German World University Service
论文部分内容阅读
Human cyclophilin 33 (hCyP33), found in 1996, consists of an RNA-binding domain in N-terminus, a cyclophilin domain in C-terminus and a connected part between the two domains. RNA-binding proteins concern functions, such as splicing, modification and transport, after transcription in eukaryotic cells. Cyclophilins (CyPs) possess enzymatic activity, namely peptidyl-proryl cis-trans isomerase (PPIase). They are involved in folding, transport and interaction of proteins. Cyclosporin A (CsA), an immunosuppressant used by organ transplantation, binds to CyPs and suppresses their enzymatic activity. However, up to now it is unknown that which cellular and physiological roles hCyP33, which possesses the above-mentioned both functions, plays. In this paper the binding specificity of hCyP33 to different cellular RNA is investigated by means of ion-exchange chromatography and affinity adsorption. The results show that it binds specifically to poly(A) tailed mRNA, namely poly(A)+RNA.
其他文献
A simple method has been proposed for the determination of clozapine (CLZ) and chlorpromazine (CPZ) in human urine by dispersive liquid-liquid microextraction (
Total organic carbon (TOC) and grain size distribution (sand,silt,and clay) in the ephemeral Mahi River (western India) sediments were measured to look at their
The desulfurizer research is focused on transition-metal oxide. The paper had discussed in depth the desulfurization activity of nano-size ZnO doped with metall
本实验截取了人降钙素受体N端胞外结构域涉及药物靶点的第20~160位氨基酸残基区域对应的编码序列,根据大肠杆菌偏爱密码子优化后,人工合成相应的基因,运用分子克隆技术将所合
The production and accumulation of propionic acid affect significantly anaerobic wastewater treatment system, but the reasons are not approached until now. Base
利用盆栽实验研究了不同程度石油污染(9 g/15 kg和18 g/15 kg,石油/土壤)土壤对甜高粱和紫花苜蓿气体交换能力、光合功能以及植物生长的影响.结果显示:相对于对照植株,受污染
As a raw material, kaolin was modified to prepare a high-performance ammonium ion-exchange material.According to cation-exchange capacity (CEC) measurement, the
Window factor analysis (WFA) is a powerful tool in analyzing evolutionary process. However, it was found that window factor analysis is much sensitive to the no
By applying co-integration analysis, the Granger causality test and an error correction model, the dependency between the energy consumption and the gross domes
Objective To study correlations of polychlorinated biphenyls (PCBs), DIOXIN, and polybrominated diphenyl ethers (PBDE) with thyroid stimulating hormone(TSH) in