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超声波处理花粉介导植物基因转化方法由山西省农业科学院生物技术研究中心发明,已被国家知识产权局授予发明专利(专利号ZL 99121152.9)。在该专利的基础上,针对玉米(Zea mays)花粉取样、保存和处理条件等因素对其体外萌发的影响进行深入研究,提出了改进玉米花粉体外萌发实验的方法。研究结果表明,在不同时期对开花的玉米进行花粉培养时所需蔗糖溶液的浓度不同;确定了玉米花粉的保存时间、条件及其对超声波处理后花粉萌发率的影响,以提高该转化方法中花粉的活力,并进一步验证了该转基因方法的可靠性;讨论了玉米花粉体外萌发的操作技巧和各因子的参数,对提高花粉介导植物基因转化效率有一定的参考价值。
Ultrasound-treated pollen-mediated plant gene transformation method invented by the Biotechnology Research Center of Shanxi Academy of Agricultural Sciences, has been granted the invention patent by the State Intellectual Property Office (Patent No. ZL 99121152.9). On the basis of this patent, the effects of sampling, preservation and treatment of pollen of Zea mays on the in vitro germination were studied in depth and the methods of improving in vitro germination of maize pollen were proposed. The results showed that the concentration of sucrose solution required for the flowering of maize during different periods was different. The storage time and conditions of maize pollen and its effect on pollen germination rate after ultrasonic treatment were determined in order to improve the transformation method Pollen vitality and further verify the reliability of the transgenic method; discusses the maize pollen in vitro germination techniques and the parameters of each factor, to improve the efficiency of pollen-mediated plant gene transformation has some reference value.