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研究了影响同源四倍体大麦花药培养的几个因素，并分析了花粉植株的变异表现，结果表明：（1）同源四倍体大麦（4n）的花药反应率比原二倍体亲本（2n）低，绿苗率却显著高于原二倍体，最高达16．07株绿苗／100枚接种花药，128株绿苗／100枚反应花药；（2）同源四倍体大麦花药培养可得到广泛的体细胞无性系变异，可为大麦育种和细胞遗传学研究提供较为丰富的基础材料，特别是四倍体经花药培养变为二倍体后，其蛋白质含量并不随着染色体倍性的恢复而降低，为饲料大麦的选育提供了一个新途径。
Several factors influencing the anther culture of autotetraploid barley were studied. The variation of pollen plants was analyzed. The results showed that: (1) The anther response rate of autotetraploid barley (4n) was higher than that of the original diploid (2n), the green plantlet rate was significantly higher than the original diploid, up to 16.07 green seedlings / 100 inoculated anthers, 128 green plantlets / 100 reaction anthers; (2) Autotetraploid barley Anther culture can obtain a wide range of somaclonal variations, which can provide rich basic materials for barley breeding and cytogenetics. In particular, after the tetraploids are transformed into diploid by anther culture, the protein content does not change with the chromosome Ploidy recovery and reduce, provide a new way for the breeding of feed barley.