室温下,水溶液中测定了Cu(Ⅱ)-L-amino acid-O-phen(1∶1∶1)体系的园二色谱。根据750～200nm光谱内的Cotton效应,确定了测量条件下络合物的绝对构型。发现在260～320nm胱氨酸和色氨酸的园二色谱带符合三齿配体结构;而亮氨酸、谷氨酸、赖氨酸和精氨酸的络合物在这段光谱内无上述现象,从而确定为二齿配体结构。 The CD spectra of Cu (II) -L-amino acid-O-phen (1: 1: 1) system were determined in aqueous solution at room temperature. Based on the Cotton effect in the 750-200 nm spectrum, the absolute configuration of the complex under the measurement conditions was determined. The circular dichroism of cystine and tryptophan at 260-320 nm was found to be in accordance with the tridentate ligand structure; whereas the complexes of leucine, glutamic acid, lysine and arginine were found to be absent in this spectrum The above phenomenon, thus identified as bidentate ligand structure.