论文部分内容阅读
为发掘果蔗抗病基因,促进果蔗抗病分子机制的研究,并为后续通过基因聚合分子育种提高果蔗品种的广谱抗性奠定基础,本研究根据已克隆的植物抗病基因保守结构域设计简并引物,采用RT-PCR方法对抗病果蔗品种黔糖5号的RNA进行扩增,共获得7条果蔗富亮氨酸重复的核苷酸结合位点(nucleotide binding site-leucine rich repeat,NBS-LRR)类抗病基因同源序列。氨基酸序列比对分析结果表明,这7条果蔗NBS-LRR类抗病基因同源序列均具有NBS典型结构域,且彼此间在氨基酸水平上表现出丰富的多态性。与7个已克隆的典型植物抗病基因同源序列构建系统进化树,7条果蔗抗病基因同源序列与基因RPM1和RPS2的亲缘关系较近,需进一步进行基因全长克隆和功能验证来确定含有这些片段的基因功能。
In order to explore the disease resistance genes of sugarcane and promote the molecular mechanism of sugarcane disease resistance, and lay the foundation for subsequent broad-spectrum resistance improvement of sugarcane varieties through gene molecular breeding. According to the conserved structure of cloned plant disease resistance genes Domain degenerate primers were designed to amplify the RNA of resistant sugarcane variety Qiangtu 5 by RT-PCR. A total of seven nucleotide leucine-rich nucleotide binding sites (nucleotide binding site- leucine rich repeat, NBS-LRR) class of resistance gene homologous sequences. The results of amino acid sequence alignment showed that the NBS-LRR homologous sequences of these 7 sugarcane plants all had NBS typical domains and showed rich polymorphism at the amino acid level with each other. The phylogenetic tree was constructed based on the homologous sequences of seven cloned typical plant disease resistance genes. The homologous sequences of seven disease resistance genes of the sugarcane are closely related to the genes RPM1 and RPS2, and need further cloning and functional verification To determine the gene function containing these fragments.