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15只家兔的颈动脉体窦神经标本,记录了33个窦神经中化学感受性传入单位的电活动。实验结果:①大部分单位放电频率较低且不规则,少部分单位的放电频率高而规则;②在灌流液内加入乙酰胆碱(ACh)20μg/ml后,16个单位的自发放电频率由1.26±0.31imp/s减至0.77±0.19imp/s(P<0.05)。③在灌流液中加入尼古丁(1、3、6、10、和15μg/ml)后,10个单位的自发放电频率,由0.60±0.21imp/s分别增加到0.96±0.21、1.19±0.30、1.24±0.29、1.48±0.41和1.82±0.39imp/s,有明显的剂量依赖性(r=0.94,P<0.001)。④阿托品(10μg/ml)可阻断ACh的作用,筒箭毒(1.0μg/ml)可阻断尼古丁作用,但对自发放电均无显著影响。上述结果提示,ACh在颈动脉体化学感受性功能方面可能仅起调质的作用。
Carotid body sinusoids in 15 rabbits recorded the electrical activity of chemosensitive afferent units in 33 sinus nerves. Experimental results: ①The discharge frequency of most units was low and irregular, the discharge frequency of a few units was high and the rules were regular. ② After the ACh 20μg / ml was added into the perfusate, the spontaneous discharge frequency of 16 units was 1. 26 ± 0.31imp / s to 0.77 ± 0.19imp / s (P <0.05). ③ After the addition of nicotine (1, 3, 6, 10, and 15μg / ml) to the perfusate, the spontaneous firing frequency of 10 units increased from 0.60 ± 0.21imp / s to 0.96 ± 0 respectively. 21, 1.19 ± 0.30, 1.24 ± 0.29, 1.48 ± 0.41 and 1.82 ± 0.39 imp / s in a dose-dependent manner (r = 0.94, P <0.001). ④ atropine (10μg / ml) can block the role of ACh, tube arrow poisoning (1.0μg / ml) can block the role of nicotine, but spontaneous discharge had no significant effect. The above results suggest that ACh may play only a role in the carotid body chemosensory function only from the role of hypochromic.