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应用电化学还原法将固定在玻碳电极表面的氧化石墨还原为石墨烯,然后利用偶联活化剂将经过氨基修饰的急性早幼粒细胞白血病(APL)PML/RARα融合基因序列探针固定到石墨烯修饰电极表面,以亚甲基蓝(MB)为电化学杂交指示剂,并由差分脉冲伏安法检测人工合成APL的PML/RARα融合基因.结果表明,石墨烯对MB的检测信号起到了很好的增敏作用,杂交前后MB还原峰电流差值与靶标链DNA浓度在5×10-10～2.5×10-9 mol/L范围内呈线性关系,检出限为8×10-11 mol/L.该方法简单、特异性好,有望用于实际样品的检测.
The graphite oxide immobilized on the surface of glassy carbon electrode was reduced to graphene by electrochemically reducing method and then the amino-modified APL PML / RARα fusion gene sequence probe was immobilized by coupling activator Graphene modified electrode surface with methylene blue (MB) as an indicator of electrochemical hybridization, and by differential pulse voltammetry APL synthetic artificial APL PML / RARα fusion gene.The results show that the graphene detection signal of MB played a very good The difference of MB reduction peak current before and after hybridization was linear with the concentration of target DNA in the range of 5 × 10-10 ~ 2.5 × 10-9 mol / L with the detection limit of 8 × 10-11 mol / L. The method is simple, specific, is expected to be used for the actual sample testing.