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目的建立RP-HPLC同时测定天麻头风灵胶囊中蒙花苷、哈巴俄苷和肉桂酸的含量。方法采用Diamonsil C18(4.6mm×200mm,5μm)色谱柱;流动相为乙腈-0.05%磷酸溶液(25∶75);流速1.0mL·min-1;柱温35℃;检测波长278nm。结果蒙花苷、哈巴俄苷和肉桂酸的质量浓度分别在11.0~110,4.60~46.0和4.20~42.0mg·L-1内与峰面积呈良好的线性关系,相关系数分别为0.9992,0.9996和0.9996;平均回收率分别为101.1%(RSD=1.9%,n=9),100.3%(RSD=1.6%,n=9)和101.8%(RSD=1.7%,n=9)。结论本方法简便、准确、重复性好,可用于天麻头风灵胶囊的质量控制。
Objective To establish an RP-HPLC method for the simultaneous determination of linarin, harpagoside and cinnamic acid in Tianmatoufengling Capsules. Methods Diamonsil C18 (4.6mm×200mm, 5μm) column was used. The mobile phase was acetonitrile-0.05% phosphoric acid solution (25∶75); flow rate was 1.0mL·min-1; column temperature was 35°C; detection wavelength was 278nm. Results The mass concentration of linicalin, harpagoside and cinnamic acid showed a good linear relationship with the peak area in the range of 11.0~110, 4.60~46.0 and 4.20~42.0mg·L-1, respectively. The correlation coefficients were 0.9992 and 0.9996 respectively. 0.9996; The average recoveries were 101.1% (RSD=1.9%, n=9), 100.3% (RSD=1.6%, n=9) and 101.8% (RSD=1.7%, n=9), respectively. Conclusion The method is simple, accurate and reproducible, and can be used for quality control of Tianma Toufengling Capsules.